Light Microscope Calibration and Test

 

Stage Micrometers, Calibration Scales / Grids

1. Stage Graticules for Microscopy

1. General information
2. S1 Stage Micrometer 10mm/0.1mm
3. S1R Stage Micrometer 10mm/0.1mm, Reflected Light
4. S2 Stage Micrometer 5mm/0.05mm
5. S4 Stage Micrometer 0.1''/0.001''
6. S4R Stage Micrometer 0.1''/0.001'', Reflected Light
7. S7 England Finder
8. S8 Stage Micrometer 1mm/0.01mm
9. S9 Stage Counting Grid 0.1mm Squares
10. S10 Stage Counting Grid 0.05mm Squares
11. S11 Stage Micrometer 0.005''/0.0001''
12. S12 Stage Micrometer 0.1mm/0.002mm
13. S16 Stage Micrometer Crossed Scales 1mm/0.01mm
14. S18 Stage Micrometer Double Graduated Metric/Imperial 25mm and 1'' scales
15. S20 Stage Micrometer Double Scales Metric/Imperial 2mm/0.01mm and 0.1''/0.0005''
16. S21 Stage Micrometer Grouped Graduations 5mm/0.5mm, 2mm/0.1mm, 0.2mm/0.01mm
17. S22 Stage Micrometer Vertical 2mm/0.01mm
18. S25 Foepple Dot Vibration Test Pattern
19. S28 Counting Grid 0.01mm Squares, 0.2x0.2mm overall
20. S29 Counting Grid 0.01mm Squares, 1.5x1.5mm overall
21. S30 Halton Finder Slide
22. S48 Stage Micrometer 1mm/0.01mm without Coverglass
23. S78 Stage Micrometer 1mm/0.01mm, Reflected Light
24. SG7 Particle Analysis Test Calibration Slide
25. S84 HSE/NPL Test Slide for Phase Contrast Calibration in Asbestos Analysis

2. Calibration Standards

1. P6 Contact Non Parallax Scale 100mm/0.1mm divisions
2. P16 Contact Non Parallax Scale 300mm/0.5mm divisions

3. Calibration Standards for Metrology

All PS range micrometer scales are engraved with a unique serial number thus satisfying the need for full traceability when supplied with a UKAS or NPL certificate of calibration

1. PGR100 Two Dimensional Grid  Ruled Area 100 x 100mm 
2. PGR300 Two Dimensional Grid Ruled Area 300x300mm
3. PS1 Micrometer Scale 10mm in 0.1mm divisions
4. PS1R Micrometer Scale 10mm in 0.1mm divisions for reflected light
5. PS4 Micrometer Scale 0.1inch in 0.001inch divisions
6. PS4R Micrometer Scale  0.1inch in 0.001inch divisions for reflected light
7. PS5 Micrometer Scale 20mm in 0.01mm divisions - brightfield
8. PS5R Micrometer Scale 20mm in 0.01mm divisions for reflected light
9. PS6R Micrometer Scale 6mm in 0.1mm divisions for reflected light
10. PS8 Micrometer Scale 1mm in 0.01mm divisions
11. PS12 Micrometer Scale 0.1mm in 0.002mm divisions
12. PS16 Crossed Micrometer Scales 1mm in 0.01mm divisions
13. PS16R Crossed Micrometer Scales 1mm in 0.01mm divisions for reflected light
14. PS20 Universal Calibration Slide for machine vision and image analysis systems. 13 different image areas.
15. PS25 Calibration Slide for Hardness Testers - Vickers/Rockwell
16. PS26 Calibration Slide for Hardness Testers - Brinell
17. PS50 Micrometer Scale 50mm in 0.1mm divisions, 75x75mm glass
18. PS52P Dual Micrometer Calibration Slide, 76mm x 25mm, positive image, 50mm in 0.1mm divisions and 2" in 5" divisions, serial
19. PS52N Dual Micrometer Calibration Slide, 76mm x 25mm,  negative image, 50mm in 0.1mm divisions and 2" in 5" divisions, serial
20. PS78 Micrometer Scale 1mm in 0.01mm divisions (for reflected light)
21. PS100 Long Scale 100mm in 0.1mm divisions
22. PS100HS MKII Long Scale 100mm in 1.0mm divisions - High Definition
23. PS100XO Long Scale 100mm in 0.1mm divisions + cross and dot series, High Definition
24. PS150 Long Scale 150mm in 0.1mm divisions
25. PS150HS MKII Long Scale 150mm in 1mm divisions - High Definition
26. PS150XO Long Scale 150mm in 0.1mm divisions + cross and dot series, High Definition
27. PS200HS MKII Long Scale 200mm in 1mm divisions - High Definition
28. PS200XO Long Scale 200mm in 0.1mm divisions + cross and dot series, High Definition
29. PS300 Long Scale 300mm in 0.1mm divisions
30. PS300HS MKII Long Scale 300mm in 1mm divisions - High Definition
31. PS300HS MKII Ultra Flat - Long Scale 300mm in 1mm divisions on Ultra Flat glass, suitable for high precision DKD certifications
32. PS300XO Long Scale 300mm in 0.1mm divisions + cross and dot series, High Definition
33. PS300XO Ultra Flat Long Scale 300mm in 0.1mm divisions + cross and dot series on Ultra Flat glass, suitable for high precision DKD certifications
34. PS500 Long Scale 500mm in 1mm divisions
35. PS1000 Long Scale 1000mm in 1mm divisions

4. High Precision Calibration Standards

Reference Stage Graticule, supplied with a certificate of calibration from NPL UKAS

5. Special Calibration Slides

1. DCI10 E112 Reference/Calibration slide
2. DCI120 Cleanliness Assessment Calibration and Reference Slide

6. Counting Chambers

1. S50 Sedgewick Rafter Counting Chamber (plastic). Includes 1 cover glass
2. S51 Coverglass for Sedgewick Rafter Cells S50 & S52
3. S52 Sedgewick Rafter Counting Chamber (glass). Includes 1 cover glass
4. S53 Sedgewick Rafter Counting Chamber (glass), plain version - no grid pattern. Includes 1 cover glass
5. REPAIR Recementing of S52 Frame
6. S60 Howard Cell for Fruit Juices
7. K20 Coverglass for Howard Cell
8. Malassez Cell
9. Thoma Cell
10. Neubauer Cell
11. Improved Neubauer Cell
12. Burker Cell
13. Nageotte Cell
14. Makler Cell
15. NEW Shukratara Cell
16. Improved McMaster Cell
17. Helber Cell
18. Evaluation Cell
19. Fertility - Semen Cell

Reticles

See reticle diameters for common microscopes - the Graticules Optics compiled list of eyepieces and microscope types from the major microscope manufacturers listed alongside the reticle sizes that fit them:

1. NE1 Horizontal Micrometer Scale 10mm/0.1mm 
2. NE120 Horizontal Micrometer Scale 20mm/0.1mm
3. NE2 Vertical Micrometer Scale 10mm/0.1mm 
4. NE5 Horizontal Micrometer Scale 5mm/0.05mm 
5. NE7 Horizontal Micrometer Scale 10mm/0.1mm with Crosslines 
6. NE7N Horizontal Micrometer Scale 10mm/0.1mm with Crossline & 10mm Square
7. NE8 Crosslines, 0.015-0.020mm line width
8. NE10 Grid pattern, 0.5mm pitch, covers whole surface
9. NE10A Indexed Grid 10 x 10 grid, 0.5mm pitch, marked 1-10 & A-J
10. NE11 Grid pattern, 1.0mm pitch, covers whole surface
11. NE11A Indexed Grid 10 x 10 grid, 1.0mm pitch, marked 1-10 & A-J
12. NE15 Chessboard pattern, 2.0mm squares, covers whole surface
13. NE17 Crossed Micrometer Scales 10mm/0.1mm 
14. NE18 Crossed Micrometer Scales 5mm/0.05mm
15. NE19 Gauge Pairs
16. NE20 Horizontal Scale 0.1" in 0.001" divisions
17. NE22 Concentric Circles, 24 circles with clear segment, 0.5mm - 12mm diameter
18. NE25 Half Protractor 10mm dia in degrees and scale 1mm/0.5mm
19. NE28 Horizontal Scale 1mm/0.01mm 
20. NE29 Whipple Grid 100 squares in 7mm area, centre square further divided into 5 x 5 grid
21. NE29B Whipple Grid 100 squares in 10mm area, centre square further divided into 5 x 5 grid
22. NE31 Horizontal Scale 0.5" length in 0.005" divisions
23. NE34 Grid pattern, 0.1mm Squares , covers whole surface
24. NE34A Indexed Grid, 10 x 10 grid, 0.1mm pitch, marked 1-10 & A-J
25. NE35 Numbered Grid 1mm Squares, each square numbered, 0-99
26. NE38 Squares 10mm, 7mm, 4mm
27. NE41 Horizontal Scale 10mm/0.05mm
28. NE42 Concentric Circles, 10 circles, 0.25mm-2.5mm diameters
29. NE43 Concentric Circles, 10 circles, 0.5mm-5mm diameters
30. NE44 Concentric Circles, 10 circles, 1mm-10mm diameters
31. NE45 Full 360 Degree Protractor 10mm dia.in degrees
32. NE47 Concentric Circles, 10 circles, 2mm-20mm diameter
33. NE48 Concentric Circles, 10 circles  1mm - 10mm diameter + graduated cross-scales, 10mm/0.1mm
34. NE50 Single Line, width 0.02mm
35. NE53 Vertical lines 0.1mm apart and horizontal line
36. NE54 Vertical Lines 0.2mm apart
37. NE56 Crossline Broken Line (Dashed)
38. NE57 Miller Square, 7 x 7 mm large square with smaller square, ratio 9:1
39. NE57C Miller Square, 8 x 8 mm large square with centred smaller square, ratio 10:1
40. NE70 Crossed micrometer scales, 0.8" scales subdivided into 2" divisions
41. NE71 Index pattern, 10mm x 10mm grid with 0.5mm squares, marked 1-20, A-T
42. NE72 Crossed micrometer scales, 20mm scales subdivided into 0.1mm divisions
43. NE77 Horizontal Micrometer Scale, 5mm/0.05mm with Crossline
44. NE81 Crossline - Line Width 0.04mm
45. NE82 Crossline - Line Width 5mm
46. NE777  Horizontal Micrometer Scale 0.5"/5" with Crossline
47. NG1 Paterson Globes/Circle
48. NG2 Original Porton Globes/Circles
49. NG5 Fairs
50. NG10 British Standard Globes & Circles BS 3625
51. NG12 New Porton Pattern Globe & Circles
52. NG14 Counting Pattern - Soil Analysis
53. NG21 Lennox for Grain Analysis
54. G22 Walton/Beckett for  Asbestos 3-1 Ratio. All with calibration factor of 4 for use with 40x objectives
55. G23 Thompson for Dust Analysis
56. G24 Walton/Beckett For Asbestos 5-1 Ratio. All with calibration factor of 4 for use with 40x objectives
57. G25 Walton/Beckett (1996). All with calibration factor of 4 for use with 40x objectives
58. NG52 Chalkley Point Array
59. MGM1 Merz for Stereology
60. NGW1 Weibel Type 1 for Stereology
61. NGW2 Weibel Type 2 for Stereology
62. GW3 Weibel Type 3 for Stereology
63. NG30 Matthews Spray Droplet
64. G41 ASTM Grain Sizing Austenite
65. G42 ASTM Grain Sizing E112
66. G44 ASTM E45
67. G45 ASTM Grain Sizing E19-46
68. G46 ASTM Grain Sizing E19-46 Root2
69. G47 Zeiss Integrating Eyepiece Disc 1
70. G48 Kotter
71. G49 Henning Reseau Pattern (Zeiss Integrating Disc 1)
72. G50 Integrating Eyepiece [simplified]
73. G54 ASTM 24 Point Circular Grid
74. G55 ASTM 25 point
75. G57 Pharmaceutical PSA Pattern
76. NG60 Grain Sizing graticule to EN10247
77. NG61 Grain Sizing graticule to ISO4967