PELCO easiSlicer™
PELCO easiSlicer™
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EB+GFAP+DAPI - 50μm
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Affordable Quality.A fresh approach to traditional standards, the PELCO easiSlicer™ brings precision vibratory tissue slicing to labs of all sizes. |
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P. Nahirney - UVic, B.C.; Cresyl Violet 50μm
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Consistent Reliability.Solid construction and exceptional stability provides exacting control of section thickness with minimal z-axis vibration. |
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C. Meshul - Portland VAMC
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Simple Versatility.Simple, effective, manual design makes the PELCO easiSlicer™ perfect for multi-user and multi-application labs. |
The PELCO easiSlicer™ introduces a new standard in vibratory slicing. It combines ease of operation, precise engineering and affordable pricing in a high quality instrument. The PELCO easiSlicer™ uses rigid construction and advanced engineering technologies to essentially eliminate backlash resulting in minimal Z-axis vibration normally associated with more expensive vibratory slicers. All operational controls are located on the front of the instrument, clearly labeled and sealed against moisture. The specimen tray is held securely in the polypropylene bath by a set of strong magnets. The bath is attached from the side with a unique spring-loaded base that locks onto the PELCO easiSlicer™ by simple pressure. The integrated bath and tray design allows them to move as a unit for sectioning thickness or height adjustment. The bath and tray can be easily separated for simple and fast clean-up, eliminating the risk of leaks and a cumbersome drain or hose.
The amplitude and speed controls are conveniently mounted in the center of the control panel for ease of operation. The instrument is supplied with a newly designed lever tightening blade holder set at a 15° cutting angle that accepts all knife thicknesses from Feather® blades to the thick Sapphire blade. Also available are blade holders for Feather® blades offered in two fixed cutting angles: 18° and 21°. The specialized design of these blade holders provides a secure and rigid grasp of the cutting blade. Feather® Blades are recommended for use with these 18° and 21° fixed cutting angle blade holders. The fixed cutting angle blade holders will accommodate other types of blades, but will not accept a Sapphire blade. The Sectioning Window Controls guide the user for precise cutting window control with indicator lights on the control panel.
The Magnifier and Light Assembly offers bright white LED lighting (no risk of high temperatures) with high or low settings, depending on the user’s need. The Magnifier can be moved out of the viewing path, if desired. The entire assembly operates smoothly on metal slides.
The large Section Thickness Knob is centrally located in the work area. The knob is labeled in microns and has a perceptible detent every 5 microns. One complete revolution of the knob is 100 microns. The entire bath and tray assembly is raised and lowered by this Section Thickness Knob. The PELCO easiSlicer™ has a Return Switch that provides the user with an option for Auto Return after each slice. In the Manual Return mode, the cutting will cease once the end of the section window has been reached. This allows the retraction of the specimen before the return of the blade in order to prevent damage to the specimen.
The PELCO easiSlicer™ comes equipped with tools and supplies to start cutting quality, consistent sections, right out of the box.
Features & Benefits
- Precision design for accurate, high quality sections with minimal z-axis vibration
- Novel bath design utilizes unique easy-locking base making it simple to clean
- Anodized specimen tray magnetically locks onto bath
- Optional magnetic base for tissue mounts available
- Cuts sections down to 20 microns
- LED lights indicate active vibratory status and cutting window boundaries
- Adjustable Blade Holder utilizes compression lever release and tightening, 15º cutting angle. Securely holds the thinnest Feather® blades to a Sapphire Blade.
- Optional Removable Blade Holders available for Feather® blades at 18º and 21º preset angles.
- Fully assembled, ready to use
- Affordable pricing
- Universal power supply 100-240V, 50/60Hz
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Novel Bath (#11010) design utilizes unique easy-locking base for secure and simple attachment; no risk of fluid leaking into the instrument. |
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Magnifier/LED Light Assembly avoids risk of high temperature, offers 2X magnification and convenient, smooth operation. |
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Removable Blade Holder (#11051-15) with innovative lever release and tightening of blades for easy knife exchange. Securely holds blades at a 15º cutting angle. Will hold most all blades for vibratory slicers from the thinnest Feather® blades to the Sapphire knife/blade. |
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Optional Removable Blade Holders with innovative tooling for secure and rigid hold of Feather® blades at 18º (#11050-18) and 21º (#11050-21) angles. |
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Convenient Blade Holder Rest (#11055) facilitates easy blade exchange. |
Optional base for tissue mounts (11014) magnetically locks into bath in place of specimen tray as shown. Four sizes of engraved tissue mounts available.
All operational controls are located on the front of the PELCO easiSlicer™, clearly labeled and sealed against moisture. Easily visible LED lights indicate active vibratory status, an exclusive safety feature, and cutting window boundaries.
PELCO easiSlicer™ Specifications
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Maximum Specimen Size
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40 x 40mm (1.58 x 1.58")
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Maximum Specimen Height
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15mm (0.6")
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Specimen Bath
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Spring-loaded; polypropylene
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Specimen Tray
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Rectangular with magnet release.
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Blade Type
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Feather® blades recommended
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Blade Angle
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15° standard; 18°, 21° available
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Sectioning Window
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10 - 40mm (0.4 - 1.58")
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Specimen Height Adjustment (Z-Axis)
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15mm (0.59") total travel in 1μm increments; tactile click stops at 5μm
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Cutting Arm Speed (Y-Axis)
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Forward: 0 - 2.0mm/sec (0 - 0.08"/sec)
Reverse: 5mm/sec (0.2"/sec) Travel: 40mm (1.58”) total |
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Vibratory Amplitude (X-Axis)
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0 - 1.00mm (0.04") total; continuously adjustable
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Cutting Arm Frequency
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50Hz ±5 factory set
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Magnification
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2X magnifier
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Illumination
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LED illumination; LOW/OFF/HIGH
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Dimensions
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535 x 300 x 290mm (21.1 x 11.8 x 11.4")
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Weight
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16.8 kg (37lbs)
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Utility Requirements
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100 - 240V; 50/60 Hz; 345VA
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Standard delivery of PELCO easiSlicer™ Vibratory Tissue Slicer (#11000) include the following accessories:
- 1x Black Anodized Tray (11020);
- 1x Bath (11010);
- 1x Lever Release Blade Holder 15° (11051-15);
- 1x Box of 10 Feather® Blades (121-9);
- 1x PELCO® Pro Specimen Retriever (101-33);
- 1x Bottle PELCO® Pro CA44 Tissue Adhesive, 20ml (10033);
- 1x PELCO easiSlicer™ Dust Cover (11060);
- 1x PELCO easiSlicer™ Blade Holder Rest (11055);
- 1x Set of Camel's Hair Brushes (11870);
- 1x Replacement Fuse (030-758)
Other accessories can be ordered separately.
Related Products
Feather® Blades
121-9
Carbon steel; sharp edges. Break in half lengthwise for use (protect fingers and use eye protection). This is the preferred cutting blade for use with the PELCO easiSlicer™. Ideally suited for use with Microslicer™, Vibratome® and other vibratory slicers. Excellent when used as is (unbroken) in McIlwain Tissue Chopper.
• Length: 42.8mm (1.685")
• Cutting Edge Length: 36mm (1.42")
• Width: 22mm (0.865")
• Thickness: 0.127mm (0.0045")
PELCO® Pro Specimen Retrievers
101-33
This is a perfect tool for retrieving free floating sections from the bath. Made of glass.
PELCO® Pro CA44 Tissue Adhesive
10033
PELCO® Pro CA44 is an instant tissue adhesive based on cyanoacrylate. Developed to glue tissue on specimen holders used in vibratory microtomes. Although the tissue adhesive is instable in boiling water, it is recommended to use #19920 PELCO® Pro Cyanoacrylate Debonder to remove or clean cured cyanoacrylate. Supplied in 20ml bottle with dispenser tip, closed with PTFE coated steel pin for optimum seal. Comparable to Loctite 404 tissue adhesive.
I - SDS (213KB PDF)
Camel Hair Brushes
11859, 11860, 11862, 11878
Useful for histology section manipulation, SEM, geology, forensic. Seamless ferrules, red enameled handles. #6 brush for larger brushing work on specimens. Measurements taken just above ferrule.
Synthetic Sable Brushes
11806...11816
For precise manipulation of TEM specimen grids and samples, dusting of SEM samples and photographic materials or photographic spotting. Synthetic sable hairs are springy and relatively fine. Length of hairs are short for better manipulation. Enameled wood handles and seamless nickel ferrules. Offered in packages of three brushes. Measurements taken just above ferrule.
Camel Hair Brush
11872
Full Body Camel Hair Brush secured by a crimped metal ferrule.
Ideal for cleaning cryostats and microtomes of debris and dust. Overall length 22.86cm (9").
Corning Costar® Tissue Culture Plates
Tissue Culture-treated Plates are designed for a wide range of applications including general cell growth experiments, cloning studies, virus isolation and in vitro testing.
Plate bottoms have a uniform thickness, allowing for distortion-free observation. Alphanumeric coordinates are placed on the same focal plane as cell growth, for convenient referencing of cell position. Gripping edges make handling easier.
All plates have non-reversible covers with condensation rings to minimize evaporation and the risk of contamination.
Plates are made of optically clear, medical-grade non-pyrogenic polystyrene, TC-treated, sterile and are used for processing of tissue on the bench or in the microwave. The corresponding Wellplate Inserts are No. 36168 for the 6 cavity, No. 36170 for the 12 cavity, and No. 36172 for the 24 cavity.
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Number of Wells |
Well Diameter |
Well Growth Area |
Total Well Volume |
Working Volume |
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| 36168-6 | 6 | 35mm | 9.5cm2 | 15ml | 2-3ml |
| 36170-12 | 12 | 22.6mm | 7ml | 1ml | |
| 36172-24 | 24 | 16mm | 1.9cm2 | 3.4ml | 0.5ml |
36168-6 6-Cavity
Tissue Culture Plate
36170-12 12-Cavity
Tissue Culture Plate
36172-24 24-Cavity
Tissue Culture Plate
SecureSeal™ Sealing Film
SecureSeal™ is a thermal adhesive sealing film which consists of 2.0 mil polyolefin film coated on one side with pressure sensitive acrylate adhesive. Designed to reduce spill-over and well to well contamination in tissue culture plates, SecureSeal™ also minimizes evaporation and vapor loss for PCR applications.
This film has low autofluorescence and is functional from -70°C to +100°C. Certified RNase, DNase and DNA-free; DMSO resistant. Available in sterile and non-sterile packages.
36167-1, 36167-2
Amplate™ Roller
Roller made of medium hard rubber secured in a heavy duty handle.
Manufactured with a comfort grip to assist in creating a perfect seal when used with SecureSeal™ Sealing Film.
36167
PELCO Prep-Eze™ Wellplate Inserts
The rectangular polypropylene Wellplate Inserts have 6, 12, or 24 wells and will fit into the Corning Costar® Tissue Culture Plates of 6/12/24 Wellplate design. A 420µm opening, polypropylene mesh is at the bottom of each of the insert wells.
The Wellplate Inserts may only be chemically sterilized.
The outside dimensions of the Wellplate Inserts are: 81.3mm W x 123.5mm L x 24mm H (3.2" W x 4.86" L x 0.88" H)
Well dim. of 6-wellplate inserts: 24.1mm dia x 22.3mm D (0.95" dia x 0.88" D)
Well dim. of 12-wellplate inserts: 14.2mm dia x 22.3mm D (0.56" dia x 0.88" D)
Well dim. of 24-wellplate inserts: 11.2mm dia x 22.3mm D (0.44" dia x 0.88" D)
Depth of well from top to bottom on all Wellplate Inserts: 17.68mm (0.696")
36168
PELCO Prep-Eze™
6-Wellplate Insert
36170
PELCO Prep-Eze™
12-Wellplate Insert
36172
PELCO Prep-Eze™
24-Wellplate Insert
PELCO® Technical Notes, PELCO Prep-Eze™ Rectangular Wellplate Inserts - Instructions (346KB PDF)
8-Section Staining Nets
Dimensions: 3-1/2" diameter x 1-1/2" high (89 x 38mm). Available with netting of 32 mesh (left) or 64 mesh (right, shown in Staining Dish 36754-60). Rod in center of net can be grasped to lift net from Staining Dish.
36154-32, 36154-64
Documentation
Documentation
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