PAP Pens for Immunohistochemical Applications & Section Adhesive Pens

    

A-PAP Pen: Liquid Blocker

ready-to-use hydrophobic barrier pens

22309, 22311

General Use: Immunohistochemistry
Surfaces: Glass

The Super PAP, Liquid Blocker Pens, are now called the A-PAP Pen (Advanced PAP Pen).

PAP Pens are used for immunohistochemical applications. The hydrophobic properties allow the user to draw barriers on the slide, in any pattern, to confine the flow of reagents to a defined area. A minimum of valuable antiserum will be used. Visible marking is light green/blue and can be removed with Xylene.

For immunohistochemistry, the PAP Pen should be applied after deparaffinization.

Directions:

1. Deparaffinize tissue sections and hydrate to water.
2. Wipe away excess liquid around the section on the glass slide with tissue paper.
3. Encircle the tissue section or draw lines on both sides of the section and let dry (10-15 seconds). Soak slides in PBS.

SDS (92KB PDF)
PELCO®Technical Notes, Super PAP Pen, Mini PAP Pen Liquid Blocker (PDF 87KB)

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Paraffin Pen: Para-Tissuer

22301

General Use: Histology
Surfaces: Glass

Adhesive pen for paraffin sections. A coating of the glass slide will prevent paraffin sections from falling off or wrinkling. Will flatten sections in room temperature water. Stable to 120° C.

PELCO®Technical Notes, Para-Tissuer, Adhesive Pen for Paraffin Sections (PDF 479KB)

MSDS (68KB PDF)

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Pen for Frozen Sections: Fro-Tissuer

22302

General Use: Cryo Sectioning
Surfaces: Glass

Adhesive pen for frozen sections. Will prevent sections from falling off or wrinkling. Effective with PAP/PAAP, ABC, LAB-SA and immunofluorescent methods. Stable up to 110°C.

PELCO® Technical Notes, Fro-Tissuer, Adhesive Pen for Frozen Sections (PDF 479KB)

MSDS (PDF 68KB)

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Tissue Capture Pen

one pen for two applications

22310

General Use: Histology
Surfaces: Glass

The new Tissue Capture Pen for use with frozen or paraffin sections. A coating of the glass slide will prevent the sections from falling off or wrinkling. Stable up to 110°C. Pen contains material for 3000-5000 applications.

PELCO® Technical Notes, Tissue Capture Pen (398KB PDF)

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Grid Coating Pen: Coat-Quick "G"

1556

General Use: TEM
Surfaces: TEM Grids - Copper, Molybdenum, Gold, Silver, Titanium, Nickel, Aluminum, Beryllium, Carbon

A light touch with this pen will provide a thin layer of adhesive on the grid. Layer will dry in 1-2 minutes at room temperature and is then ready to receive the sections. Pen may also be used as pretreatment before coating grids with formvar or other thin films.

SDS (121KB PDF)

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ImmEdge™ Barrier Pen

a hydrophobic barrier pen

22312

General Use: Immunohistochemistry, In situ Hybridization
Surfaces: Glass

The ImmEdge™ Pen, a hydrophobic barrier pen for immunohistochemistry and in situ hybridization detection methods, is designed for use with frozen or paraffin-embedded tissue sections mounted on glass slides. The ImmEdge™ Pen provides a water repellent barrier that keeps staining reagents localized on the tissue sections, prevents mixing of reagents when differentially staining two sections on the same slide, and allows the use of less reagents per section.

The boundary deposited by the ImmEdge™ Pen is lightly colored and easily visible. It is insoluble in alcohol and acetone, but is removed by most clearing agents. The ImmEdge™ Pen is compatible with all commonly used enzyme or fluorescent-based detection systems.

SDS (PDF 749KB)