Immunolabeling / Confocal Microscopy Application Kit

The PELCO ColdSpot^® Pro

36116-10

designed as a temperature controlled surface
will enhance most tissue processing steps

The PELCO ColdSpot^® Pro is designed for control of the environment inside the PELCO BioWave^® Pro and Pro+ microwave systems. The water within the PECLO ColdSpot^® Pro circulates through the internal Load Cooler within the BioWave^® system or through the external PELCO SteadyTemp™ Pro chiller. This provides consistent temperature control at the specimen level as well as eliminating the standing wave pattern within the microwave cavity.

The PELCO ColdSpot® Pro is designed for microwave processing techniques that benefit from an efficient heat transfer between the PELCO ColdSpot® surface and the sample. This product design provides excellent temperature control for electron microscopy tissue processing, immunolabeling and in situ hybridization protocols. The glass surface dissipates heat quickly and efficiently. This design creates a flat surface across the entire glass top enabling a wide range of usage with consistency. The temperature of the water inside this PELCO ColdSpot® should not exceed 60°C (140° F) because the thermal properties of the plastic and the adhesive will be compromised at higher temperatures. All PELCO® Microwave processors can be set to maintain the temperature below this critical point. Designed for use with 3536 PELCO® Vacuum Chamber.

Glass plate area dimensions: 223 W x 291mm D (8.78 W x 11.34" D)
Overall dimensions: 267 W x 381 D x 108mm H (10.5 W x 15 D x 4.25" H)

PELCO^® EM Pro Microwave Vacuum Chamber

3536

The PELCO® EM Pro Vacuum Chamber is made from microwave-transparent polypropylene and designed to reach a maximum vacuum of 20" of Hg. The PELCO® EM Pro Vacuum Chamber works in conjunction with the internal vacuum pump in the PELCO BioWave® Pro and Pro+ microwave systems. The vacuum chamber can run a continuous vacuum or utilize a vacuum cycle, depending on the application and need of the user.

Features

• Made of chemically resistant, microwave-transparent polypropylene and designed for vacuum down to 20" of Hg (1 torr/1mm Hg) and temperatures up to 70°C.
  
  
• Designed either to run a continuous vacuum during microwave processing or a vacuum cycle.
  
  
• Improves fixative penetration and ultrastructural preservation during microwave-assisted chemical fixation.
  
  
• Improves resin infiltration in the microwave and greatly reduces the turnaround times when compared to established microwave protocols.
  
  
• Can be used in or outside of the PELCO BioWave^® Pro Microwave Tissue Processor.

3536-10

Top Plate with Temperature Probe Port (optional). Note that the Temperature Probe with cable connection for the PELCO BioWave® Pro Microwave Tissue Processor is inserted (also optional)
See below for all accessories.

PELCO® EM Pro Vacuum Chamber shown with optional Top Plate with temperature probe port and optional Temperature Probe in place.
See Table 1 The effect of Microwaves/Vacuum on the Sample Processing Times for Electron Microscopy