Citifluor Antifade Mounting Medium

Citifluor provides a range of antifadent mounting media which greatly reduces the fading of the fluorescence from FITC, rhodamine, Hoechst, Alexa, Green Fluorescent Protein, cyanine dyes, Texas Red, and phycoerythins. A range of antifadent mountants are available in either glycerol based or glycerol free solutions. Citifluor mountant can either be permanent (hardening) or not (non-hardening) depending on your labeling requirements.

The use of fluorescent labels and markers is now widespread and immuno-fluorescence, which relies on the use of antibodies tagged with labels such as fluorescein, is a well-established technique.

A common problem associated with the microscopical examination of these materials is that the illumination used for stimulating the fluorescence also causes degradation of the label and this causes fading of fluorescence. This also is a problem when trying to visualize materials having a low level of labeling. The photofading of labeled materials can be retarded by the use of the Citifluor mountants.

Antifadent (AF) Solutions
The antifadent solutions AF-1, AF-2 and AF-3 were specifically developed for use with FITC polyclonal antibodies but their use is now far more widespread. They show improved signal preservation with many other fluorochromes including rhodamines and DAPI. AF4 contains the antifadent propyl gallate, which is particularly suitable for use with DAPI and Alexa dye-stained materials, as well as FITC-labelled materials.

AF-1 - Glycerol-phosphate buffered solution containing antifadent for use with labeled tissue and dead cells. Available in two sizes.

I - SDS (46KB PDF) for 19470, 19470-1     

I - SDS (110KB PDF) for 19472, 19472-1     

I - SDS (315KB PDF) for 19476, 19491    

I - SDS (111KB PDF) for 19492     

I - SDS (109KB PDF) for 19493    

I - SDS (48KB PDF) for 19494     

I - SDS (109KB PDF) for 19495  

I - SDS (111KB PDF) for 19496     

I - SDS(106K PDF) for 19474, 19474-1      

I - SDS (47KB PDF) for 19490     

I - SDS (69KB PDF) for 19497     

I -  SDS (90KB PDF) for 19498     

C - SDS for Citifluor AF100 Antifadent Solution, 25ml (47KB PDF)

U - SDS for Citifluor CFPVOH Solution, 5ml (44KB PDF)    

AF100 and CFPVOH contain anti-fading solution. Suitable for preventing fading of dyes such as DAPI which have the tendency to disperse in glycerol based mounting solutions.