ANTcryo on Gold Grids

Amorphous nickel-titanium perforated foils (nitinol) on gold or copper mesh for cryo-EM. They are characterized by defined hole sizes (round, size/spacing), excellent conductivity, and user-friendly handling. The advantages include improved resolution, faster sample preparation, and higher data acquisition efficiency. 

The films are on the matte side (grey/black), the reverse side is metallic and shiny (gold or copper). To hydrophilize the metallic perforated films, we recommend the Pelco easiGlow (15mA, 30-60s). 

Sample concentration using apoferritin as an example: 1 mg/ml to 1.5 mg/ml.

🔹 Technical specifications

• Material: Amorphous Nickel Titanium (NiTi/Nitinol)
• Carrier material: Gold or Copper mesh
• Hole pattern: Round holes with defined diameter and spacing
• Surface:
       o Matte side (grey/black) = active film side
       o Back side metallic shiny (gold or copper colored)
• Hydrophilization: Recommendation Pelco easiGlow (15 mA, 30–60 s)
• Example sample concentration: Apoferritin 1.0–1.5 mg/ml

• Packaging unit: 5 pieces

🔹 Main advantages

1. Improved resolution of the reconstruction
       o Minimized electron charging & reduced beam-induced motion (BIM).
2. Faster sample preparation
       o Lower adsorption capacity, more uniform particle distribution.
3. More efficient data collection
       o Higher particle density per hole, fewer micrograms required.
4. Easy integration
      o Compatible with all common cryo-EM workflows.