PELCO BioWave® Pro+ Electron Microscopy Application Kit

Table 1: The Effect of Microwaves/Vacuum on the Sample Processing Times for Electron Microscopy

Processing Step	Routine Microwave Processing 1	Vacuum Microwave Processing 2	Routine Processing
1. Primary Fixation (aldehyde)	.....10 minutes	.....6 minutes	.....1 hour
2. Buffer Rinse	.....6 minutes	.....4 minutes	.....0.5 hours
3. Secondary Fixation (osmium)	.....10 minutes	.....6 minutes	.....1 hour
4. Dehydration (acetone/ethanol)	.....7 minutes	.....7 minutes	.....2 hours
5. Resin Infiltration	.....50 minutes	.....8 minutes	.....18 hours
6. Tissue to Embedding Capsules	.....15 minutes	.....15 minutes	.....0.25 hours
7. Resin Polymerization	.....45-75 minutes	.....45-75 minutes	.....18 hours
TOTALS	.....~170 minutes	.....~120 minutes	.....~2,400 minutes

Note: we recommend using the new PELCO BioWave® Pro+ Microwave Tissue Processor with built-in manual or programmable Vacuum Pump.
If not available, we recommend the 3436 Diaphragm Vacuum Pump to provide vacuum to this vacuum chamber.

1/4" ID Silicone Vacuum Hose

92490-253, 92490-256

Thick walled silicone vacuum hose with 1/4" (6.35mm) inside diameter and 1/2" (12.7mm) outside diameter. Sturdy, yet flexible material with wall thickness of 1/8" (3.2mm), will not collapse under vacuum. Sold in lengths of 3 and 9 ft.

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Capsule Preparation Station

36131-6

The Capsule Preparation Station is designed for the size 00 embedding capsules as an aid for filling and orientation for polymerization. The prep station is made from polypropylene that allows for easy resin clean-up.

This Preparation Station is a capsule loading platform to maintain cleanliness from epon liquid spillovers from the above Microwave Capsule Holder 36131-2. This pre-loading station avoids a potential situation of water contamination in the microwave polymerization procedure. The 36131-6 permits the technician/scientist to pre-load 00 capsules. Capsules are then transferred to 36131-2 for the polymerization step in the microwave.

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 Microwave Polymerization System

Designed for resin polymerization using the 36131 or 36131-2 Capsule Holders in the PELCO BioWave^® Microwave Tissue Processor

The Microwave Polymerization System contains all the components necessary for polymerization of resin within the BioWave^® Pro and Pro+ microwave systems. The Microwave Polymerization System is compatible with epoxy and LR White resins.

Microwave Polymerization System
shown with the Polymerization Stand (36133-20)
and PELCO® Microwave Capsule Holder (36131-2)
on the PELCO ColdSpot® Pro (36116-10)

During microwave-assisted resin polymerization the microwave polymerization system is placed on the Microwave Polymerization Stand on the PELCO ColdSpot® Pro. The tray is filled with water to cover the 36131 or 36131-2 capsule holders. The insulator pad is placed on the surface of the water and the lid placed on the tray. There are two holes in the lid so that the temperature probe can be inserted on either side to monitor heating rates. This new system controls the rate of heating and the temperature maximum (100°C) without the loss of water during the polymerization process. Microwave wattage will control the heating rate which ensures a quality polymerization each run.

Microwave Polymerization System
shown expanded, with the optional
PELCO® Microwave Capsule Holder (36131-2)

Microwave Polymerization Stand

36133-20

The Polymerizations Stand (36133-20) is a foam block used to elevate the Microwave Polymerization System (36133) above the PELCO ColdSpot^® during resin polymerization. This elevation allows for controlled heating of the water in the Microwave Polymerization System. The Stand is microwave transparent and provides a stable base for the Polymerization System.

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PELCO^® Prep-Eze™

36157, 36158

The PELCO Prep-Eze™ specimen holders are designed for microwave processing. The sturdy PTFE design is offered in 6-well and 12-well configurations. Each kit contains a specimen holder, ID mat for each design, and a set of polypropylene petri dishes.

PELCO Prep-Eze™ Holders

The PELCO Prep-Eze™ Holder accomodates processing 6-12 specimen batches. The unique design provides simpler handling and reduction in the volume of chemicals needed. The PELCO Prep-Eze™ fits with the #36135 Polypropylene Petri Dishes.

The outside dimensions of the holders are: 50.8mm diameter x 19mm High (44.5mm High with handle), (2" dia. x 0.75" H [1.75" H with handle]).

No. 36157-1 has 6 wells, 12.7mm diameter x 17mm deep (.5" dia. x .67" D).
No. 36158-1 has 12 wells, 9.5mm diameter x 17mm deep (.375" dia. x .67" D).
The mesh openings are 420µm.
The small hole can be used to hold the temperature probe for specimen level temperature control.

36157-1

36157-3

36158-1

36158-3

Numbered mats, available for both sizes, are plastic laminated to resist chemicals and may be written on with a dry erase marker. With the holder placed on the mat, easy identification of specific wells is possible. The kits includes one specimen holder, one ID mat and 20 polypropylene petri dishes (58 x 15mm, Prod. No. 36135) suitable for use in the microwave.

Polypropylene Petri Dishes

36135, 36135-5

 

Polypropylene Petri Dishes are microwave transparent and sturdy. These dishes can be coupled with the PELCO Prep-Eze™ Specimen Holders for microwave processing.

Bottom inside dimensions: 50mm dia. x 12mm deep
Lid inside dimensions: 56mm dia. x 10mm deep

PELCO Prep-Eze™ Specimen Dishes

The PELCO Prep-Eze™ Specimen Dish Kits come with a rectangular polypropylene tray that have 6, 12, or 24 wells which fit 6, 1.5, or 0.5mL polyethylene specimen dishes respectively. They can be used for many steps of specimen processing, staining, and fixation. The unique design provides simpler handling, reduction in the volume of chemicals needed, and separation of samples from one another. As such, they are particularly useful for the labeling of free-floating sections, on the bench or in the microwave.

The removable dishes allows for any number of specimen dishes to be used at a given time depending on the number of samples to be processed. Once processing is completed, used specimen dishes can be discarded and replaced (specimen dish refills available).

The Specimen Dish Kits may only be chemically sterilized. All components are suitable for use in microwave processing.

The outside dimensions of the Wellplate Inserts are: 81.3 W x 123.5 L x 6.5mm H (3.2 W x 4.86 L x 0.26" H)

Kit Components:
#36180 – one 6-well tray, 25 Specimen Dishes (6mL)
#36181 – one 12-well tray, 50 Specimen Dishes (1.5mL)
#36182 – one 24-well tray, 100 Specimen Dishes (0.5mL)

36180
PELCO Prep-Eze™
6ml Specimen Dish Kit

36181
PELCO Prep-Eze™
1.5ml Specimen Dish Kit

36182
PELCO Prep-Eze™
0.5ml Specimen Dish Kit

36180-25, 36181-50, 36182-100
Specimen Dish Refills

Specimen Dish Dimensions (mm)

	A	B	C	D	E	G	H
6ml Specimen Dish	35	30	32	30.6	28.4	13.8	14
1.5ml Specimen Dish	21	17.4	18.8	18	16.4	8.8	9.2
0.5ml Specimen Dish	16	13.3	14.4	12.8	11.6	6.8	7

 

PELCO Prep-Eze™ Wellplate Inserts

The rectangular polypropylene Wellplate Inserts have 6, 12, or 24 wells and will fit into the Corning Costar® Tissue Culture Plates of 6/12/24 Wellplate design. A 420µm opening, polypropylene mesh is at the bottom of each of the insert wells.

The Wellplate Inserts may only be chemically sterilized.

The outside dimensions of the Wellplate Inserts are: 81.3mm W x 123.5mm L x 24mm H (3.2" W x 4.86" L x 0.88" H)

Well dim. of 6-wellplate inserts: 24.1mm dia x 22.3mm D (0.95" dia x 0.88" D)
Well dim. of 12-wellplate inserts: 14.2mm dia x 22.3mm D (0.56" dia x 0.88" D)
Well dim. of 24-wellplate inserts: 11.2mm dia x 22.3mm D (0.44" dia x 0.88" D)
Depth of well from top to bottom on all Wellplate Inserts: 17.68mm (0.696")

36168
PELCO Prep-Eze™
6-Wellplate Insert

36170
PELCO Prep-Eze™
12-Wellplate Insert

36172
PELCO Prep-Eze™
24-Wellplate Insert

PELCO® Technical Notes, PELCO Prep-Eze™ Rectangular Wellplate Inserts - Instructions (346KB PDF)

 

Corning Costar® Tissue Culture Plates

Tissue Culture-treated Plates are designed for a wide range of applications including general cell growth experiments, cloning studies, virus isolation and in vitro testing.

Plate bottoms have a uniform thickness, allowing for distortion-free observation. Alphanumeric coordinates are placed on the same focal plane as cell growth, for convenient referencing of cell position. Gripping edges make handling easier.

All plates have non-reversible covers with condensation rings to minimize evaporation and the risk of contamination.

Plates are made of optically clear, medical-grade non-pyrogenic polystyrene, TC-treated, sterile and are used for processing of tissue on the bench or in the microwave. The corresponding Wellplate Inserts are No. 36168 for the 6 cavity, No. 36170 for the 12 cavity, and No. 36172 for the 24 cavity.

3.8cm2

	Number of Wells	Well Diameter	Well Growth Area	Total Well Volume	Working Volume
36168-6	6	35mm	9.5cm2	15ml	2-3ml
36170-12	12	22.6mm	7ml	1ml
36172-24	24	16mm	1.9cm2	3.4ml	0.5ml

 

36168-6 6-Cavity
Tissue Culture Plate

36170-12 12-Cavity
Tissue Culture Plate

36172-24 24-Cavity
Tissue Culture Plate

SecureSeal™ Sealing Film

SecureSeal™ is a thermal adhesive sealing film which consists of 2.0 mil polyolefin film coated on one side with pressure sensitive acrylate adhesive. Designed to reduce spill-over and well to well contamination in tissue culture plates, SecureSeal™ also minimizes evaporation and vapor loss for PCR applications.

This film has low autofluorescence and is functional from -70°C to +100°C. Certified RNase, DNase and DNA-free; DMSO resistant. Available in sterile and non-sterile packages.

36167-1, 36167-2

Amplate™ Roller

Roller made of medium hard rubber secured in a heavy duty handle.

Manufactured with a comfort grip to assist in creating a perfect seal when used with SecureSeal™ Sealing Film.

36167

Individual Wells

Great for processing individual specimens or keeping specimens separate from others. Same wells as used in the Wellplate Inserts above but on an individual basis. Made entirely from polypropylene. Available in three sizes, all with 420µm openings in the screen.

36169, 36171, 36173

Dimensions of the Individual Wells:

	Outside Diameter	Inside Diameter	Deep	Opening
Individual Well 6 #36169	31.7mm	24.1mm	22.3mm	420µm
Individual Well 12 #36171	19.25mm	14mm	22.3mm	420µm
Individual Well 24 #36173	14.55mm	11.2mm	22.3mm	420µm

 

8-Section Staining Nets

Dimensions: 3-1/2" diameter x 1-1/2" high (89 x 38mm). Available with netting of 32 mesh (left) or 64 mesh (right, shown in Staining Dish 36754-60). Rod in center of net can be grasped to lift net from Staining Dish.

36154-32, 36154-64

Pyrex® Staining Dish

4" diameter x 2" high (100mm diameter x 50mm H), for use with above Staining Nets.

36754-60

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PELCO® Microwave Microcentrifuge Tubes PTFE Holder

PELCO® Microwave Microcentrifuge Tube Holder in PTFE is designed for use in the PELCO BioWave® Pro and Pro+ microwave systems. The holder can accommodate 18, 1.5ml microcentrifuge tubes.

 

PELCO® Microwave Capsule Holder with Lid

The improved PELCO® Microwave Capsule Holder has three design features that set it apart from the previous design.
"Stops" on the legs to keep the capsule base level during loading
A hole with a stop in the base to allow the 00 embedding capsule to be set in place easily
The new capture top that has a recess to keep the embedding capsule lid in place throughout the polymerization process in the microwave

36131-2 Un-assembled

36131-2 Assembled

Flat Bottom Capsules

For embedding specimens requiring flat or en-face orientation in capsule configuration. Inside dimensions are 00 size: 7.92mm dia x 18.87mm deep (0.312 x 0.743"). Polypropylene (up to 100°C). Outside diameter 9.6mm.

133-P

PELCO® Microwave Microcentrifuge Tube Holders

Made from PTFE, the holder for microcentrifuge tubes is designed to hold 18 conventional 2ml polyethylene microcentrifuge tubes. Tubes shown not included.

These tube holders may be used in a water bath or on the PELCO ColdSpot® Pro during processing.

36134

PELCO® Microwave Macrocentrifuge Tube Holders

The PTFE holder for 5ml macrocentrifuge tubes will hold 12 tubes. Tubes shown not included.

These tube holders may be used in a water bath or on the PELCO ColdSpot® Pro during processing.

36134-1

1.5ml Microcentrifuge Tubes, Clear

The 1.5ml microcentrifuge tubes are manufactured from polypropylene and able to be autoclaved. These tubes are also microwave transparent. The tubes feature a frosted label area for sample identification and volume graduations of 0.1, 0.5, 1.0 and 1.5ml. The microcentrifuge tubes have a snap cap for secure closure but are also syringe pierceable. These microcentrifuge tubes offer convenience and versatility for various utilizations in histology, immunology and scientific research.

20836, 20838

1.5ml Microcentrifuge Tubes, Black

The 1.5ml microcentrifuge tubes are composed of polypropylene and are completely autoclavable as well as microwave transparent. The opaque black provides protection for light sensitive samples. The microcentrifuge tubes offer a frosted label area for writing or moisture resistant labels. The tubes contain volume graduations of 0.1, 0.5, 1.0 and 1.5mls. The tight-fitting snap cap offers convenience and safety in storage as well as a syringe-pierceable alternative. These microcentrifuge tubes provide versatility and convenience in many facets of histology, immunology and scientific research.

20836-B

1.5ml Microcentrifuge Tubes, Assorted Colors

These 1.5 ml microcentrifuge tubes in assorted colors consist of polypropylene, are microwave transparent and autoclavable. The frosted label provides are for specimen identification. The tubes are labeled in graduated volumes of 0.1, 0.5, 1.0 and 1.5 ml. The tight-fitting snap cap offers secure closure and syringe insertion ability. The assorted colors of the 1.5 ml microcentrifuge tubes provide assistance in organization and reference for multiple samples. These tubes offer versatility in specimen handling in histology, immunology and scientific research.

20840

5ml Snap Cap Macrocentrifuge Tubes, Assorted Colors

• Provides convenience of 1.5 ml microcentrifuge tubes in a tube that holds larger volumes
• Attached Snap Cap
• Pierceable cap for needle or syringe
• Dual graduation scales in increments of 0.5ml and 0.2ml
• Temperature range of -86°C to +80°C
• Seven colors available: natural, amber, blue, black, green, red, yellow, assorted
• Polypropylene (PP)

12944-1...9

5ml Locking Macrocentrifuge Tubes, Natural

• Locking Snap Cap preventing accidental opening
• Suitable for centrifugation, boiling, incubation, freezing and sample storage
• Optimally clear USP Class VI medical grade polypropylene (PP)
• Manufactured in Class 100,000 clean room
• Certified to be free from RNase, DNase,human DNA, pyrogens, PCT inhibitors and ATP
• Tubes feature graduations, writing area, peirceable cap, are autoclavable and packaged in zip re-closable bags

12944

5ml Screw Cap Macrocentrifuge Tubes

• Single turn threaded caps seal easily and securely
• Graduated in 0.5ml increments
• Available sterile in bags or foam racks and non-sterile with tubes and caps packed separately
• Temperature range of -86°C to 80°C
• For low temperature sample storage, general laboratory uses, or boiling procedures
• Polypropylene (PP) with polyethylene (PE) screw caps
• Use with #1062 MagFuge® Centrifuge.

12944-10, 12944-11, 12944-12, 12944-14

Temperature Restrictive Temperature Probe with PTFE Coating

This PTFE-coated probe is supplied with the PELCO BioWave® Pro+ and is a replacement probe for all the previous PELCO® Microwave systems.

It is used to monitor and/or maintain preset temperature maximums during microwave processing. The temperature probe is accurate to ±1°C (from 0°C to 100°C) and will maintain ±1°C in water and most fixative solutions. The 10cm probe is protected with a PTFE coating for decalcification and special stain applications. It has a 40cm long lead and is designed to fit into Product No. 36138 PTFE Probe Stand. This stand has a 12cm long arm that can be adjusted up and down over an 18cm range to allow precise location of the probe into processing vessels or a container. The Product No. 3536 PELCO® EM Microwave Vacuum Chamber is also designed for use with this temperature probe.

36145-T & 36138